Microbiome


Equivolumetric Protocol Generates Library Sizes Proportional to Total Microbial Load in 16S Amplicon Sequencing

Library sizes obtained by 16S gene sequencing data are usually seen as arbitrary. But are they? Can we make them meaningful, both biologically and mathematically? Yes, we can.

One year cross-sectional study in adult and neonatal intensive care units reveals the bacterial and antimicrobial resistance genes profiles in patients and hospital surfaces

Bacteria are ubiquitous in hospital environments, in many cases threatening infection control. In this study, we use next-generation sequencing to track down these bacteria in intensive care units (ICUs) from a Brazilian hospital for a one-year period, covering environmental samples, hospital staff, and patients. We keep track of neonatal and adult ICUs and report abundance and diversity results as well as microbial resistance profiles.